Role of myeloperoxidase at chronic inflammations - in vivo studies

Role of Myeolperoxidase at Chronic Inflammations - in vivo studies

Aminophenyl fluorescein is a non-fluorescent fluorescein derivative which, upon oxidation by hypochlorous acid (HOCl), is oxidized to fluorescein. Therefore APF can be used to specifically detect the chlorinating activity of myeloperoxidase (MPO), i.e. the formation of HOCl by two-electronic oxidation of chloride (Cl-). After successful application of APF to isolated MPO and purified neutrophils this staining method was optimized to determine MPO activity in blood micro-samples.

This blood analysis method was subsequently applied to several animal experiments: In a mouse model for allergic rhinitis the amount of neutrophils in the blood of the animals was correlated with the chlorinating activity of the MPO. In a further study chronic rheumatoid arthritis was induced in rats by using pristane injection. Afterwards recurrent blood sampling was used to monitor the MPO activity in the blood and correlate it with the disease symptoms.

In cooperation with Dr. Sandra Franz we are currently developing a method to analyse the chlorinating MPO activity in tissue samples obtained e.g. from a wound model of diabetic mice. These studies may lead to the development of new diagnostic tools for the investigation of chronic inflammatory diseases.

Team Members

Dr. Jörg Flemmig

Collaborations

Dr. Franziska Lange
Fraunhofer Institute for Cell Therapy and Immunology (IZI)
Leipzig, Germany

Dr. Sandra Franz
University of Leipzig
Leipzig, Germany

letzte Änderung: 10.07.2017