Soluble Protein - Ligand Interactions

Soluble Protein-Ligand Interactions

In biological systems, regulatory proteins are essential for a wide range of cellular activities based on interactions with the local environment. Therefore, detailed knowledge of protein−ligand (or protein−protein) interaction is required to understand biological processes. A profound knowledge of these interactions is a crucial prerequisite to translate our research into drug design. Using solution-state NMR spectroscopy and other biophysical methods such as CD, UV/Vis and fluorescence spectroscopy, we analyze structures of small molecules and proteins, as well as protein-ligand interaction and their dynamics, e.g., of cytokines /proteases in complex with their ligands.  

 

Standard techniques in our lab include:

  • Recombinant Expression, refolding and purification of isotopically labeled (e.g. 13C, 15N) protein
  • several solution NMR methods (e.g. 1H-15N-HSQC titration, pseudo contact shifts (PCS), saturation transfer difference (STD), assignment of proteins, … )

 

 

Key Publications

  • A. Penk, L. Baumann, D. Huster, S. A. Samsonov. NMR and molecular modeling reveal specificity of the interactions between CXCL14 and glycosaminoglycans. Glycobiol. 2019, 29, 715−725.
  • G. Künze, S. Köhling, A. Vogel, J. Rademann, D. Huster. NMR characterization of the binding properties and conformation of glycosaminoglycans interacting with interleukin-10. J Biol Chem. 2016, 219, 3100–3113.
  • A. Pichert, S. A. Samsonov, S. Theisgen, L. Thomas, L. Baumann, J. Schiller, A. Beck-Sickinger, D. Huster, M. T. Pisabarro. Characterization of the interaction of interleukin-8 with hyaluronan, chondroitin sulfate, dermatan sulfate and their sulfated derivatives by spectroscopy and molecular modeling.  Glycobiol. 2012, 22, 134–145.

Team

  • Christian Schulze
  • Mathias Bosse
  • Dr. Benedikt Schwarze
  • Dr. Anja Penk

 

Collaborations

  • Dr. Sergey A. Samsonov, Faculty of Chemistry, University of Gdánsk, Poland
  • Prof. Dr. Jörg Rademann, Faculty of Pharmacy, Freie Universität Berlin
  • Dr. Sven Rothemund, Core Unit Peptide, Leipzig University
  • Prof. Dr. Annette G. Beck-Sickinger, Institute of Biochemistry, Leipzig University

Funding

Transregio-SFB TRR 67, project A6

DFG project HU 720-19-1

letzte Änderung: 16.07.2020