NanoBioDetect

 Addressing the fundamental issues for nanomaterial toxicology, risk assessment and regulatory testing requires establishing novel and innovative high-resolution dosimetry methods, which are especially suitable for nanomaterials.

The following label-free techniques for dosimetry and characterization of authentic nanomaterials in-vitro and in-vivo will be applied to address these problems: Ion Beam Microscopy (IBM), Confocal Raman Microspectroscopy (CRM), Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) and Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS). The joint approach provides a unique opportunity for development and adaptation of innovative, quick, low-cost and spatially resolved 2D/3D state-of-the-art procedures for quantitative particle detection in culture cells and organs on the cellular and subcellular level. To optimize the procedure for the quantitative detection of NPs suitable particle standards mimicking the biological environment will be developed and certified by means of which CRM, LA-ICP-MS and ToF-SIMS can be calibrated.

Furthermore the correlation between the actual intracellular dose in identified affected cells and the locally triggered physiological/toxic response is to be investigated.